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intercepttm pbs blocking buffer  (LI-COR)


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    Structured Review

    LI-COR intercepttm pbs blocking buffer
    Intercepttm Pbs Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/intercepttm+pbs+blocking+buffer/Intercept+(PBS)+Blocking+Buffer/pm41858052-41-29-34
    Average 98 stars, based on 1113 article reviews
    intercepttm pbs blocking buffer - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Membrane:

    Article Title: Alterations in the p53 isoform ratio govern breast cancer cell fate in response to DNA damage.
    Article Snippet: .. The membrane was blocked with Casein Blocking Buffer (Millennium Science, Mulgrave VIC, Australia) or InterceptTM PBS Blocking Buffer (LI-COR Biosciences, Lincoln, NE, USA) at room temperature for 1 h. The following primary antibodies were diluted in blocking buffer: CM-1 (pan-p53 polyclonal antibody, which recognises epitopes located between amino acids 1 and 393), 1 μg/ml; KJC40, 2.5 μg/ml (The University of Dundee, Scotland); DO-1, 1 μg/ml (Merck); 7F5, 1 μg/ml (Cell Signaling Technology); mouse GAPDH, 1 μg/ml (Calbiochem, San Diego, CA, USA; #CB1001); rabbit GAPDH, 1 μg/ml (Abcam, Melbourne, VIC, Australia; #ab128915), and added to the membrane overnight (4 °C, rocking). .. Diluted secondary antibodies (1–5 μg/ml; LI-COR Biosciences; #926-32210 and #926-68023) in blocking buffer were added and allowed to bind on a rocker for at least 1 h at room temperature.

    Blocking Assay:

    Article Title: Alterations in the p53 isoform ratio govern breast cancer cell fate in response to DNA damage.
    Article Snippet: .. The membrane was blocked with Casein Blocking Buffer (Millennium Science, Mulgrave VIC, Australia) or InterceptTM PBS Blocking Buffer (LI-COR Biosciences, Lincoln, NE, USA) at room temperature for 1 h. The following primary antibodies were diluted in blocking buffer: CM-1 (pan-p53 polyclonal antibody, which recognises epitopes located between amino acids 1 and 393), 1 μg/ml; KJC40, 2.5 μg/ml (The University of Dundee, Scotland); DO-1, 1 μg/ml (Merck); 7F5, 1 μg/ml (Cell Signaling Technology); mouse GAPDH, 1 μg/ml (Calbiochem, San Diego, CA, USA; #CB1001); rabbit GAPDH, 1 μg/ml (Abcam, Melbourne, VIC, Australia; #ab128915), and added to the membrane overnight (4 °C, rocking). .. Diluted secondary antibodies (1–5 μg/ml; LI-COR Biosciences; #926-32210 and #926-68023) in blocking buffer were added and allowed to bind on a rocker for at least 1 h at room temperature.

    Article Title: Low, plasma level‑informed native curcumin concentrations fail to induce cell death in human lung and colorectal cancer cells.
    Article Snippet: .. Complete Mini Protease Inhibitor Cocktail Tablet was obtained from Roche; the BCA Protein Assay Kit from Thermo Fisher Scientific; 4–20% SDS-PAGE Gels from Bio-Rad; PVDF membranes from Merck; and InterceptTM (PBS) Blocking Buffer from LI-COR Biosciences. ..

    Article Title: Mevalonate kinase-deficient THP-1 cells show a disease-characteristic pro-inflammatory phenotype.
    Article Snippet: As loading control, membranes were incubated with a primary antibody against a-tubulin (1:2,000, Sigma-Aldrich, T6199). .. For visualization, we used the secondary antibodies IRDye 800CW goat anti-rabbit, IRDye 800CW goat anti-mouse or IRDye 680RD donkey anti-mouse (LI-COR Biosciences) at a 1:10,000 dilution in a 1:1 dilution of TBS-T and InterceptTM (PBS) blocking buffer (LICOR Biosciences) with 0.02% SDS. ..

    Article Title: Identification of FDA-approved drugs that increase mevalonate kinase in hyper IgD syndrome.
    Article Snippet: .. Secondary antibodies used were IRDye 800CW goat anti-rabbit, IRDye 800CW goat anti-mouse or IRDye 680RD donkey antimouse (LI-COR Biosciences) at a 1:10 000 dilution in a 1:1 dilution of PBS and InterceptTM (PBS) blocking buffer (LI-COR Biosciences) with 0.1% Tween-20 and 0.02% SDS. .. After washing with PBS-T, membranes were scanned using the Odyssey Infrared Imaging System (LI-COR Biosciences).

    Article Title: Refeeding with different levels of dietary carbohydrate modulates epigenetic stability through global DNA (de)methylation and histone modifications in juvenile and adult Nile tilapia ( Oreochromis niloticus )
    Article Snippet: .. Primary antibodies were used at a 1:1,000 dilution in InterceptTM (PBS) Blocking Buffer (LICORbio, USA; cat. no. 927–70001): anti-H3K4me3 (polyclonal; Diagenode, Belgium; cat. no. C15410003), anti-H3K9me3 (polyclonal; Diagenode, Belgium; cat. no. C15410056), anti-H3K9ac (polyclonal; Diagenode, Belgium; cat. no. C15410177), and anti-H3 (polyclonal; Abcam, UK; cat. no. ab1791). .. The secondary antibody, goat anti-rabbit IgG H&L (HRP) (polyclonal; Abcam, UK; cat. no. ab205718), was used at a 1:10,000 dilution in the same blocking buffer.

    Article Title: Mevalonate kinase-deficient THP-1 cells show a disease-characteristic pro-inflammatory phenotype
    Article Snippet: As loading control, membranes were incubated with a primary antibody against α-tubulin (1:2,000, Sigma-Aldrich, T6199). .. For visualization, we used the secondary antibodies IRDye 800CW goat anti-rabbit, IRDye 800CW goat anti-mouse or IRDye 680RD donkey anti-mouse (LI-COR Biosciences) at a 1:10,000 dilution in a 1:1 dilution of TBS-T and InterceptTM (PBS) blocking buffer (LI-COR Biosciences) with 0.02% SDS. ..

    Article Title: Sonlicromanol’s active metabolite KH176m normalizes prostate cancer stem cell mPGES-1 overexpression and inhibits cancer spheroid growth
    Article Snippet: Total proteins were separated by 10% or 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Amsterdam, the Netherlands). .. Membranes were blocked with InterceptTM (PBS) Blocking Buffer (LI-COR, Lincoln, the United States) for 1 h at room temperature and then incubated with anti-mPGES-1 (1:5000, Agrisera, Vännäs, Sweden) and anti-β-actin (1:10000, Sigma-Aldrich, Zwijndrecht, the Netherlands) used as a loading control at 4°C overnight. ..

    Article Title: Alterations in the p53 isoform ratio govern breast cancer cell fate in response to DNA damage
    Article Snippet: A total of 30-50 μg of protein extracts, obtained using 1% NP-40 lysis buffer [50 mM Tris/HCl, 150 mM NaCl, 1% NP-40, pH 8.0, 19 Mini Complete Protease Inhibitor EDTA-free cocktail tablets (Roche Diagnostics, 11836153001)], were resolved on 8– 12% SDS/PAGE gels and transferred onto nitrocellulose membranes. .. The membranes were blocked in InterceptTM PBS Blocking Buffer (LI-COR Biosciences, 927- 70001, Lincoln, NE, USA) and probed using primary antibody anti-p53 (pantropic), clone DO-1 (Merck Millipore, MABE327, Burlington, MA, USA) at a concentration of 1 μg mL 1 (detects p53a, b and c) and KJCA40 (provided by Dr Jean-Christophe Bourdon) at a concentration of 2.5 μg mL 1 (detects all D40p53 variants). .. GAPDH was used as a loading control (anti-rabbit: Abcam, ab128915; anti-mouse: Calbiochem, CB1001).

    Protease Inhibitor:

    Article Title: Low, plasma level‑informed native curcumin concentrations fail to induce cell death in human lung and colorectal cancer cells.
    Article Snippet: .. Complete Mini Protease Inhibitor Cocktail Tablet was obtained from Roche; the BCA Protein Assay Kit from Thermo Fisher Scientific; 4–20% SDS-PAGE Gels from Bio-Rad; PVDF membranes from Merck; and InterceptTM (PBS) Blocking Buffer from LI-COR Biosciences. ..

    Bicinchoninic Acid Protein Assay:

    Article Title: Low, plasma level‑informed native curcumin concentrations fail to induce cell death in human lung and colorectal cancer cells.
    Article Snippet: .. Complete Mini Protease Inhibitor Cocktail Tablet was obtained from Roche; the BCA Protein Assay Kit from Thermo Fisher Scientific; 4–20% SDS-PAGE Gels from Bio-Rad; PVDF membranes from Merck; and InterceptTM (PBS) Blocking Buffer from LI-COR Biosciences. ..

    SDS Page:

    Article Title: Low, plasma level‑informed native curcumin concentrations fail to induce cell death in human lung and colorectal cancer cells.
    Article Snippet: .. Complete Mini Protease Inhibitor Cocktail Tablet was obtained from Roche; the BCA Protein Assay Kit from Thermo Fisher Scientific; 4–20% SDS-PAGE Gels from Bio-Rad; PVDF membranes from Merck; and InterceptTM (PBS) Blocking Buffer from LI-COR Biosciences. ..

    Incubation:

    Article Title: Sonlicromanol’s active metabolite KH176m normalizes prostate cancer stem cell mPGES-1 overexpression and inhibits cancer spheroid growth
    Article Snippet: Total proteins were separated by 10% or 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Amsterdam, the Netherlands). .. Membranes were blocked with InterceptTM (PBS) Blocking Buffer (LI-COR, Lincoln, the United States) for 1 h at room temperature and then incubated with anti-mPGES-1 (1:5000, Agrisera, Vännäs, Sweden) and anti-β-actin (1:10000, Sigma-Aldrich, Zwijndrecht, the Netherlands) used as a loading control at 4°C overnight. ..

    Control:

    Article Title: Sonlicromanol’s active metabolite KH176m normalizes prostate cancer stem cell mPGES-1 overexpression and inhibits cancer spheroid growth
    Article Snippet: Total proteins were separated by 10% or 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a polyvinylidene difluoride (PVDF) membrane (Merck Millipore, Amsterdam, the Netherlands). .. Membranes were blocked with InterceptTM (PBS) Blocking Buffer (LI-COR, Lincoln, the United States) for 1 h at room temperature and then incubated with anti-mPGES-1 (1:5000, Agrisera, Vännäs, Sweden) and anti-β-actin (1:10000, Sigma-Aldrich, Zwijndrecht, the Netherlands) used as a loading control at 4°C overnight. ..

    Concentration Assay:

    Article Title: Alterations in the p53 isoform ratio govern breast cancer cell fate in response to DNA damage
    Article Snippet: A total of 30-50 μg of protein extracts, obtained using 1% NP-40 lysis buffer [50 mM Tris/HCl, 150 mM NaCl, 1% NP-40, pH 8.0, 19 Mini Complete Protease Inhibitor EDTA-free cocktail tablets (Roche Diagnostics, 11836153001)], were resolved on 8– 12% SDS/PAGE gels and transferred onto nitrocellulose membranes. .. The membranes were blocked in InterceptTM PBS Blocking Buffer (LI-COR Biosciences, 927- 70001, Lincoln, NE, USA) and probed using primary antibody anti-p53 (pantropic), clone DO-1 (Merck Millipore, MABE327, Burlington, MA, USA) at a concentration of 1 μg mL 1 (detects p53a, b and c) and KJCA40 (provided by Dr Jean-Christophe Bourdon) at a concentration of 2.5 μg mL 1 (detects all D40p53 variants). .. GAPDH was used as a loading control (anti-rabbit: Abcam, ab128915; anti-mouse: Calbiochem, CB1001).



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    Image Search Results


    Journal: The EMBO Journal

    Article Title: BBS8-dependent ciliary Hedgehog signaling governs cell fate in the white adipose tissue

    doi: 10.1038/s44318-025-00524-y

    Figure Lengend Snippet: Reagents and tools table

    Article Snippet: Membranes were blocked with InterceptTM Blocking Buffer (in PBS, LI‐COR Biosciences, #927‐70001) supplemented with Tween® 20 (Sigma, #P9416).

    Techniques: Mouse Assay, Recombinant, Sequencing, Red Blood Cell Lysis, Marker, Blocking Assay, Software, Staining, Bicinchoninic Acid Protein Assay, Reverse Transcription, SYBR Green Assay, cDNA Synthesis, DNA Library Preparation, Flow Cytometry, Microscopy, Laser-Scanning Microscopy, Imaging, Real-time Polymerase Chain Reaction